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CD47+ Target Cell

CBP74124

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I. Background
CD47 (also known as Rh-associated protein, GP42, integrin-associated protein (IAP), and neurophilin,) is an immunoglobulin-like protein that interacts with its receptor, Signal-regulatory protein alpha (SIRPα), on macrophages. This binding interaction regulates transmigration, oxidative burst cytokine production, and phagocytosis, creating a “don’t eat me” signal. CD47 is ubiquitously expressed on the surface of normal cells, but is overexpressed in numerous cancer cells, where it is thought to contribute to the resistance of tumors to phagocyte-dependent clearance.
 
II. Introduction
Expressed gene: CD47
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Rh-associated protein, GP42, integrin-associated protein (IAP), and neurophilin
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+5ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Representative Data

Figure 1. Recombinant CHO-cells constitutively expressing CD47.

Figure 2. Dose response of SIRPɑ Blocking Antibodies in SIRPɑ Effector Reporter Cells With CD47+ Target Cells.

Figure 3. Dose response of CD47 Blocking Antibody in SIRPɑ Effector Reporter Cells(C11) With CD47+ Target Cells.

 

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